Biomarkers in Tumor Samples From Younger Patients With Neuroblastoma

NCT01493830 — Completed, 5 participants, Children's Oncology Group

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Proposed Studies on the Role of Growth Factor Receptor Trafficking in Neuroblastoma Pathogenesis

Registry	NCT01493830
Status	Completed
Study type	Observational
Enrollment	5 (Estimated)
Sponsor	Children's Oncology Group (Network); collaborators: National Cancer Institute (NCI)
Conditions	Neuroblastoma
Interventions	fluorescence in situ hybridization, gene expression analysis, microarray analysis, mutation analysis, protein analysis, immunohistochemistry staining method, laboratory biomarker analysis, microscopy (Genetic, other)
Dates	start 2011-12-01, primary completion 2016-07-01, first posted 2011-12-16, last updated 2016-07-11

-Summary

RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This research trial studies biomarkers in tumor samples from younger patients with neuroblastoma.

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+Detailed description

OBJECTIVES:

* To determine the expression of UBE4B, hrs, and members of the ubiquitination protein complex in previously banked primary neuroblastoma tumor samples from the Children's Oncology Group.
* To determine the correlation of expression levels and cellular localization of the UBE4B and hrs proteins with growth factor-receptor tumor cell-surface expression.
* To determine the 1p36 deletion status by fluorescence in situ hybridization (FISH) analysis and their association with expression levels of UBE4B and hrs in previously banked primary neuroblastoma tumor samples from the Children's Oncology Group.

OUTLINE: Banked tumor tissue samples are analyzed for expression of UBE4B, hrs, members of the ubiquitination protein complex, growth factor receptors, and 1p36 deletion status by immunohistochemistry (IHC), fluorescent microscopy, and fluorescence in situ hybridization (FISH).

+Arms and interventions

fluorescence in situ hybridization (Genetic)
gene expression analysis (Genetic)
microarray analysis (Genetic)
mutation analysis (Genetic)
protein analysis (Genetic)
immunohistochemistry staining method (Other)
laboratory biomarker analysis (Other)
microscopy (Other)

+Outcome measures

Primary outcomes

Expression of UBE4B, hrs, and members of the ubiquitination protein complex
Expression levels and cellular localization of the UBE4B, hrs, and growth factor receptors
1p36 deletion status and its association with UBE4B

+Eligibility

Sex: All, ages: any to 120 Years

DISEASE CHARACTERISTICS:

* Tumor tissue microarrays from the Children's Oncology Group neuroblastoma tumor bank

PATIENT CHARACTERISTICS:

* Not specified

PRIOR CONCURRENT THERAPY:

* Not specified

+Investigators

Peter Zage, MD, PhD, Principal investigator, Texas Children's Cancer Center