Evaluation of Thioldisulfide Balance in Cervical Preinvasive Lesions

To date various oxidative stress paramaters were studied in patients with cervical preinvasive lesions, however dynamic thiol disulfide hemostasis has not been previously investigated. The thiol-disulfide balance in the circulation will be measured by the automated system created by Erel \& Neselioglu(1).

References 1- Erel O, Neselioglu S. A novel and automated assay for thiol/ disulphide homeostasis. Clin Biochem 2014;47:326-32. doi:10.1016/ j.clinbiochem.2014.09.026.

• Disulphide(μmol/L) (Diagnostic test), also known as Native thiol (μmol/L), Total thiol (μmol/L), Nativethiol/totalthiol(%), Disulfide/nativethiol(%), Disulfide/totalthiol(%): Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with sphectrophotometry
• Total Oxidant Capacity(μmolH2O2Equiv/L) (Diagnostic test), also known as Total Antioxidant Capacity(mmolTroloxEquiv/L), Oxidative stress Index (Arbitrary Unite): Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with sphectrophotometry

Primary outcomes

• serum native thiol level [baseline (preoperative in the morning or preprandial for healthy volunteers)]: serum native thiol level in μmol/L
• serum disulphide level [baseline (preoperative in the morning or preprandial for healthy volunteers)]: serum disulphide level in μmol/L
• serum total thiol level [baseline (preoperative in the morning or preprandial for healthy volunteers)]: serum total thiol level in μmol/L
• the disulphide/native thiol ratio [baseline (preoperative in the morning or preprandial for healthy volunteers)]: the disulphide/native thiol ratio X (100 )
• disulphide/total thiol ratio [baseline (preoperative in the morning or preprandial for healthy volunteers)]: disulphide/total thiol ratio X (100 )
• native thiol/total thiol ratio [baseline (preoperative in the morning or preprandial for healthy volunteers)]: native thiol/total thiol ratio X (100 )

Secondary outcomes

• total antioxidant capacity (TAC) [baseline (preoperative in the morning or preprandial for healthy volunteers)]: TAC in mmol trolox equivalent/L
• total oxidant capacity (TOC) [baseline (preoperative in the morning or preprandial for healthy volunteers)]: TOC in mmol H2O2 equivalent/L
• Oxidative stress index (OSI) [baseline (preoperative in the morning or preprandial for healthy volunteers)]: OSI in mmol trolox/L.

Sex: Female, ages: 18 Years to any, accepts healthy volunteers

Inclusion Criteria:

• Histopathologically confirmed diagnosis of LGSIL or HGSIL

Exclusion Criteria:

* pulmonary disease
* pulmonary hypertension
* cardiac dysfunction
* renal dysfunction
* liver disease
* chronic ishemia
* systemic inflammation
* other cervical biopy results than LGSIL or HGSIL
* concomitant malignancy
* patients who use vitamin A,C or E (antioxidant vitamins)
* patients who use smoke • patients who use drink alcohol
* patients who use addictive for any drug