Engineering A Hyper-Adherent E. Coli Nissle Probiotic Strain That Reduces Intestinal Carriage of Salmonella Pathogens in Poultry

M Aaron Baxter; Kelsey Greenwood; Kristi L Anderson; Steve A Carlson; Bradley D Jones;

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ABSTRACT

Poultry meat and eggs are major sources of foodborne illness, due to carriage of these bacterial pathogens in the intestinal microbiome of chickens, which is an ongoing food safety issue throughout the world. Reducing or eliminating the carriage of in poultry would significantly improve food safety and reduce cases of human salmonellosis. This study represents a new approach to reduce or eliminate carriage of in poultry flocks. We have created an Nissle 1917 probiotic strain that expresses a factor with high levels of adherence. The Nissle 1917 scaffold has been used for many years as a probiotic to treat human intestinal disorders and is designated GRAS by the FDA. Our probiotic strain displays high levels of adherence to host cells, in fact, higher levels of adherence than a strain carrying the same type 1 fimbrial genes. We demonstrate that the probiotic strain significantly outcompetes pathogenic strains for adherence to tissue culture cells (more than 100-fold; >2 logs), and in vivo experimental challenges revealed that the probiotic strain mediates a significant exclusion (more than a 10-fold reduction; >1 log) of from the intestines of broilers, layers, and turkeys.

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