Increased Atherosclerosis in HIV-Infected Humanized Mice Is Caused By A Single Viral Protein, Nef

The Journal of infectious diseases  ·  2024

Yongsen Wang; Beda Brichacek; Larisa Dubrovsky; Tatiana Pushkarsky; Kyle Korolowicz; Olga Rodriguez; Yichien Lee; Marta Catalfamo; Chris Albanese; Anastas Popratiloff; Dmitri Sviridov; Michael Bukrinsky;

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ABSTRACT

Antiretroviral therapy suppresses human immunodeficiency virus (HIV) replication, reverses immunodeficiency, and reduces AIDS-related symptoms, but non-AIDS comorbidities like cardiovascular diseases remain a major challenge for people with HIV (PWH). The pathogenic mechanisms driving these comorbidities are poorly understood. We previously showed that the HIV protein Nef contributes to chronic inflammation in PWH. Here, we explored Nef's role in HIV-associated atherosclerosis using a novel model: HIV-infected humanized mice expressing a gain-of-function mutant of proprotein convertase subtilisin/kexin type 9 (PCSK9) and fed a high-fat diet. Comparing atherosclerosis in uninfected mice to those infected with Nef-positive or Nef-deficient HIV-1, we found that Nef exacerbates atherosclerotic changes by increasing inflammation. These results identify Nef as a key driver of HIV-related atherosclerosis and provide a platform for testing therapeutic interventions targeting Nef to mitigate cardiovascular risks in PWH.

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