Shared and Distinct Transcriptomic Cell Types Across Neocortical Areas

Nature  ·  2018  ·  Impact 9

Bosiljka Tasic; Zizhen Yao; Lucas T Graybuck; Kimberly A Smith; Thuc Nghi Nguyen; Darren Bertagnolli; Jeff Goldy; Emma Garren; Michael N Economo; Sarada Viswanathan; Osnat Penn; Trygve Bakken; Vilas Menon; Jeremy Miller; Olivia Fong; Karla E Hirokawa; Kanan Lathia; Christine Rimorin; Michael Tieu; Rachael Larsen; Tamara Casper; Eliza Barkan; Matthew Kroll; Sheana Parry; Nadiya V Shapovalova; Daniel Hirschstein; Julie Pendergraft; Heather A Sullivan; Tae Kyung Kim; Aaron Szafer; Nick Dee; Peter Groblewski; Ian Wickersham; Ali Cetin; Julie A Harris; Boaz P Levi; Susan M Sunkin; Linda Madisen; Tanya L Daigle; Loren Looger; Amy Bernard; John Phillips; Ed Lein; Michael Hawrylycz; Karel Svoboda; Allan R Jones; Christof Koch; Hongkui Zeng;

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ABSTRACT

The neocortex contains a multitude of cell types that are segregated into layers and functionally distinct areas. To investigate the diversity of cell types across the mouse neocortex, here we analysed 23,822 cells from two areas at distant poles of the mouse neocortex: the primary visual cortex and the anterior lateral motor cortex. We define 133 transcriptomic cell types by deep, single-cell RNA sequencing. Nearly all types of GABA (γ-aminobutyric acid)-containing neurons are shared across both areas, whereas most types of glutamatergic neurons were found in one of the two areas. By combining single-cell RNA sequencing and retrograde labelling, we match transcriptomic types of glutamatergic neurons to their long-range projection specificity. Our study establishes a combined transcriptomic and projectional taxonomy of cortical cell types from functionally distinct areas of the adult mouse cortex.

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