Periodontal Pathogens and Atherom Plaque The Number of Mitochondrial DNA Copies

NCT07165054 — Completed, 51 participants, Istanbul Saglik Bilimleri University

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Registry	NCT07165054
Status	Completed
Study type	Observational
Enrollment	51 (Actual)
Sponsor	Istanbul Saglik Bilimleri University (Other)
Conditions	Periodontal Inflammation, Biofilm, Endothelial Dysfunction, Oral Bacteria
Interventions	atheroma plaque, Dental plaque (Other)
Dates	start 2025-04-10, primary completion 2025-11-01, completion 2025-12-01, first posted 2025-09-10, last updated 2026-01-30
Locations	1 site in Turkey (Türkiye)

-Summary

Periodontitis is a chronic inflammatory disease of the tooth-supporting tissues characterized by the constant interaction of microorganisms in subgingival plaque and host defense mechanisms. Periodontitis is considered a focus of infection caused by oral bacteria, bacterial byproducts, or inflammatory mediators that can interact with other parts of the body via the bloodstream.

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+Detailed description

Periodontal bacteria produce endotoxins in the form of lipopolysaccharide (LPS). Circulating LPS reacts with Toll-like receptors, promoting the production of reactive oxygen species and phagocytosis in the intima layer. Oxidative stress has been reported to be associated with mtDNA copy number.

+Arms and interventions

Arm	Type	Interventions	Description
Patients diagnosed with endarterectomy		Other: atheroma plaque; Other: Dental plaque

atheroma plaque (Other): Before the endarterectomy procedure, samples will be collected for study from some of the atheroma plaques routinely taken for pathology from patients.
Dental plaque (Other): Subgingival plaque samples will be collected from patients before the endarterectomy procedure.

+Outcome measures

Primary outcomes

Bacterial frequency (%) [Months 6-9 of the study]: The percentage of bacteria in the red complex in atheroma plaque and dental plaque is evaluated using polymerase chain reaction methods.

Secondary outcomes

Changes in mtDNA copy number (number) [Months 6-9 of the study]: Evaluation by means of quantitative polymerase chain reaction of mtDNA copy number in atheroma plaque
periodontal healths (mm) [8 months]: Evaluation by means of periodontal indices such as PI, GI, BOP, PD.

+Eligibility

Sex: All, ages: 25 Years to 90 Years

Inclusion Criteria:

* Diagnosed with endarterectomy
* Not receiving chemotherapy
* Not having received this treatment before

Exclusion Criteria:

* Having systemic infection
* Patients with severe heart disease

+Locations (1)

Facility	City	Country	Status
Health Sciences University, Hamidiye Faculty of Dentistry, Department of Periodontology	Istanbul	Turkey (Türkiye)

+Publications

Results publication: Toraman A, Saglam E, Toraman E, Ketenciler S, Koseoglu S. Red-Complex Bacterial DNA in Carotid Atheroma of Dentate and Long-Term Edentulous Patients and Its Association With Mitochondrial DNA Copy Number. Oral Dis. 2026 Jul 16. doi: 10.1111/odi.70431. Online ahead of print. PMID 42464421