Hydrogen-Rich Water and Resting Metabolism in Young Adults

NCT07098221 — Not applicable, Not yet recruiting, 24 participants, University of Novi Sad

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Effects of Hydrogen-Rich Water on Resting Metabolism in Healthy Young Adults: A Randomized, Double-Blind, Placebo-Controlled Crossover Trial

Registry	NCT07098221, acronym H2REST
Status	Not yet recruiting
Phase	Not applicable
Study type	Interventional; randomized, crossover, masking: triple, basic science
Enrollment	24 (Estimated)
Sponsor	University of Novi Sad (Other)
Conditions	Healthy Subjects or Volunteers
Interventions	Molecular Hydrogen, Placebo Comparator (Dietary supplement)
Dates	start 2025-09-01 (estimated), primary completion 2025-12-01 (estimated), completion 2025-12-01 (estimated), first posted 2025-08-01, last updated 2025-08-01

-Summary

The H2REST trial is a randomized, double-blind, placebo-controlled crossover study investigating the acute effects of a single dose of hydrogen-rich water (HRW) on resting metabolism in healthy young adults. Participants complete two testing sessions-receiving either HRW or placebo in a randomized order-followed by standardized metabolic testing. Primary outcomes include resting energy expenditure, respiratory exchange ratio, and substrate utilization measured via indirect calorimetry. The study aims to assess whether acute HRW intake can modulate metabolic rate and fuel preference at rest, and to explore inter-individual variability in response.

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+Arms and interventions

Arm	Type	Interventions	Description
ARM1: 250 ml of Hydrogen Rich Water	Experimental	Dietary Supplement: Molecular Hydrogen	Hydrogen-producing tablets: Tablets designed to dissolve in water and release molecular hydrogen gas, creating hydrogen-rich water that can be consumed to potentially provide antioxidant and anti-inflammatory benefits.
ARM 2: 250 ml of Placebo Water (Non-hydrogen-producing tablets)	Placebo comparator	Dietary Supplement: Placebo Comparator	Non-hydrogen-producing tablets, identical in appearance and taste to the active supplement

Molecular Hydrogen (Dietary supplement): Participants in this arm will consume a single 250 mL dose of hydrogen-rich water containing approximately 1.6-2.0 ppm of dissolved molecular hydrogen (H₂), The HRW will be ingested following an overnight fast, and resting metabolism will be measured approximately 30-60 minutes post-consumption using indirect calorimetry.
Placebo Comparator (Dietary supplement): Non-hydrogen-producing tablets, identical in appearance and taste to the active supplement

+Outcome measures

Primary outcomes

Resting Energy Expenditure (REE) [Participants will attend three study visits, with each intervention separated by a 7-day washout period to minimize carry-over effects. During each visit, measurements will be conducted approximately 30-60 minutes post-intervention.]: The measurements will be obtained during each study visit. Since this is an interventional study assessing the acute effects of molecular hydrogen, measurements will be collected three times: At baseline, without any intervention (pre-familiarization), After a single dose of molecular hydrogen, and After placebo administration. The period between each intervention will be seven days, serving as a washout period to prevent carry-over effects. All participants will be instructed to maintain their regular diet and to avoid alcohol, caffeine-containing beverages, and strenuous physical activity for at least 24 hours prior to each visit. Measure Type: Continuous (kcal/day) Method: Indirect calorimetry Description: To assess the effect of acute ingestion of hydrogen-rich water versus placebo on resting energy expenditure in healthy young adults.

+Eligibility

Sex: All, ages: 18 Years to any, accepts healthy volunteers

Inclusion Criteria:

* Aged 18-35 years
* BMI between 18.5 and 25.0 kg/m²
* Apparently healthy, with no history of chronic disease
* Non-smoker, not currently using nicotine-containing products
* Able to provide informed consent
* Willing to refrain from alcohol, caffeine, and strenuous exercise for 24 hours before each study visit
* Able to comply with study procedures, including fasting and attending all study visits

Exclusion Criteria:

* Diagnosed metabolic, cardiovascular, respiratory, gastrointestinal, or endocrine disorders
* Use of dietary supplements or medications affecting metabolism (within the past 2 weeks)
* Allergy or intolerance to components of the hydrogen or placebo beverages
* Pregnant or lactating
* Shift work or irregular sleep schedule
* Participation in another clinical study within the past 30 days