Evaluation of the Salivary-free Amino Acids Levels (Glycine , Proline , Lysine , and Alanine ) With Caries Activity

NCT06953557 — Not yet recruiting, 80 participants, Al-Mustansiriyah University

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Evaluation of the Salivary-free Amino Acids Levels With Caries Activity

Registry	NCT06953557
Status	Not yet recruiting
Study type	Observational
Enrollment	80 (Estimated)
Sponsor	Al-Mustansiriyah University (Other)
Conditions	Dental Caries
Interventions	To compare the effect of salivary glycine , proline , lysine and alanine concentration between participants with high caries activity and participants with negative caries activity (Diagnostic test)
Dates	start 2025-04-30 (estimated), primary completion 2025-05-30 (estimated), completion 2025-06-30 (estimated), first posted 2025-05-01, last updated 2025-05-01

-Summary

This study will be aimed to investigated the correlation between levels of salivary amino acids (glycine , proline , lysine , alanine ) in the saliva of healthy children with and without active carious lesions and to estimate the predictive capacity of caries susceptibility

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+Detailed description

80 healthy children (8-10 years old) will be recruited from primary schools of Baghdad city/ Iraq, will be classified according to their level of caries activity (high caries activity, n = 40; negative caries activity, n = 40). Total stimulated and unstimulated saliva will be collected to estimate the caries activity by using the Snyder test, and Amino acid analysis of the saliva will be performed by reversed phase - high performance liquid chromatography (RP-HPLC), respectively.

+Arms and interventions

To compare the effect of salivary glycine , proline , lysine and alanine concentration between participants with high caries activity and participants with negative caries activity (Diagnostic test): The investigators will measure Salivary-free amino acids by using reverse phase high performance liquid chromatography

+Outcome measures

Primary outcomes

Salivary-free amino acid analysis [Baseline :unstimulated saliva collection for salivary glycine , proline , lysine and alanine measurement]: Unstimulated saliva collection for salivary free amino acids analysis

+Eligibility

Sex: All, ages: 8 Years to 10 Years, accepts healthy volunteers

Inclusion Criteria:

* Children aged (8,9,10) years with different caries
* Absence of signs of gingivitis or periodontal disease.

Exclusion Criteria:

* Children out the range of age
* Children with any systemic discases.
* Children with appliances in the mouth.
* Children with appliances in the mouth.
* Children with antibiotic treatment or any other drug over the last two weeks. B