Physical Activity Level and Sleep Quality in Type 2 Diabetes

NCT06602778 — Completed, 80 participants, Selcuk University

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Association of Physical Activity Level and Sleep Quality with Oxidative Stress and Exercise-related Biomarker Levels in Type 2 Diabetes

RegistryNCT06602778
StatusCompleted
Study typeObservational
Enrollment80 (Actual)
SponsorSelcuk University (Other)
ConditionsType 2 Diabetes Mellitus (T2DM), Sleep, Biomarkers / Blood, Physical Activity Level
InterventionsBiomarkers / blood, Questionnaire (Other)
Datesstart 2024-09-20, primary completion 2024-10-20, completion 2025-02-25, first posted 2024-09-19, last updated 2025-03-19
Locations1 site in Turkey (Türkiye)

Related Papers Related Patents Related Grants Related Experts
All trials for Type 2 Diabetes Mellitus (T2DM) All trials for Sleep All trials of Biomarkers / blood All trials of Questionnaire

-Summary

In this study, we investigated the effects of physical activity level and sleep quality level on total antioxidant level (TAS), total oxidant level (TOS), Malondialdehyde (MDA) and Superoxide Dismutase (SOD) which are markers of oxidative stress, irisin, fibronectin type III-protein 5 (FNDC5).

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+Arms and interventions

ArmTypeInterventionsDescription
T2DM-BMI0Other: Biomarkers / blood; Other: Questionnaire
T2DM-BMI1Other: Biomarkers / blood; Other: Questionnaire
T2DM-BMI2Other: Biomarkers / blood; Other: Questionnaire
T2DM-BMI3Other: Biomarkers / blood; Other: Questionnaire
  • Biomarkers / blood (Other): Analysis of routine biochemical parameters
  • Questionnaire (Other): Physical activity level will be assessed using the International Physical Activity Questionnaire Short Form (IPQI) and sleep quality will be assessed using the Pittsburgh Sleep Quality Index (PSQI). The data to be obtained from blood will be obtained by selecting blood samples that will be sent to biological waste.

+Outcome measures

Primary outcomes

  • biomarkers derived from blood sera [10 Minutes]: blood glucose, total cholesterol, triglycerides, LDL and HDL analyzes
  • ELISA method of oxidative stress markers [10 minutes]: All reagents of the ELISA kits of the oxidative stress markers TAS, TOS, MDA and SOD will be brought to room temperature. Standards will be prepared according to the protocol and added to a 96-well plate with the samples. Buffer solution will be added to each well and incubated for 90 min at room temperature. After each step, necessary washes will be performed and finally chromogenic agents will be added and read spectrophotometrically. Although the timing and additions may vary according to the parameter, in general the procedure is as indicated. Each sample will be run 2 times for both TAS and TOS
  • Analysis of exercise-related markers by ELISA method [10 minutes]
  • International Physical Activity Questionnaire [5 minutes]
  • Pittsburgh Sleep Quality Index [5 minutes]

+Eligibility

Sex: All, ages: 30 Years to 65 Years

Inclusion Criteria:

* Being between 30-65 years old
* Volunteering to participate in the study
* Being literate
* Being diagnosed with Type 2 Diabetes

Exclusion Criteria:

* Having a physical or mental illness that prevents communication
* malignant disease,
* infectious disease,
* chronic obstructive pulmonary disease,
* liver disease,
* patients with kidney disease and joint or muscle disease will not be included

+Locations (1)

FacilityCityCountryStatus
Selcuk UniversityKonya, SelcukluTurkey (Türkiye)