Analysis of Protein Expression in Serum of KOA Subjects

NCT06553313 — Not applicable, Unknown, 50 participants, China Medical University Hospital

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Analysis of Protein Expression in Serum of Subjects With Knee Arthritis Control Group and Evaluation by Meridian and Pulse Wave Energy

Registry	NCT06553313
Status	Unknown
Phase	Not applicable
Study type	Interventional; na, single group, masking: none, basic science
Enrollment	50 (Estimated)
Sponsor	China Medical University Hospital (Other)
Conditions	Knee Osteoarthritis
Interventions	Micro-Western Array (Device)
Dates	start 2024-08-04, primary completion 2025-07-23 (estimated), completion 2025-07-23 (estimated), first posted 2024-08-14, last updated 2024-08-14
Locations	1 site in Taiwan

-Summary

In the previous study "Using omics technology to explore the mechanism of acupuncture treatment of different acupoints of knee osteoarthritis: a randomized controlled trial (CMUH109-REC3-113)", we found that acupuncture for knee osteoarthritis(KOA) significantly reduces inflammation, apoptosis, and metabolism-related serum protein profiles. However, due to the lack of serum protein data from the control group, it is impossible to establish standard values for comparison. To analyze the protein expression in the serum of KOA control group subjects, we also measured the spectral energy values of the radial artery pulse wave obtained from the pulse sphygmograph and the meridian energy values obtained from Ryodoraku. The results obtained can be compared with previous studies, thus this experiment was designed.

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+Arms and interventions

Arm	Type	Interventions	Description
the control group of knee arthritis	Other	Device: Micro-Western Array	Monitoring the stability of protein expression, pulse wave and meridian energy in the serum of the KOA control group (participants without knee pain).

Micro-Western Array (Device), also known as Enzyme-linked immunosorbent assay, pulse sphygmograph, Ryodoraku: The subjects first took the energy measurement of the pulse sphygmograph and Ryodoraku, rested for 20 minutes, and then repeated the measurements again. In order to comply with the acupuncture treatment course was 5 weeks in the previous study, we have two blood draw point. Each subject's blood was drawn for the first time, and then for the second time in 5 weeks later. Each subject drew 10ml of blood each time. The blood samples are analyzed by Micro-Western Array (MWA) and Enzyme-linked immunosorbent assay (ELISA) to obtain the serum protein content value of healthy individuals.

+Outcome measures

Primary outcomes

Micro-Western Array and Enzyme-linked immunosorbent assay [the first and the fifth weeks]: Obtaining the serum protein content value

Secondary outcomes

pulse sphygmograph and Ryodoraku [the first and the fifth weeks]: the energy measurement of pulse and meridians

+Eligibility

Sex: All, ages: 50 Years to any, accepts healthy volunteers

Inclusion Criteria:

* Adults over 50 years old without knee pain

Exclusion Criteria:

1. Clinical diagnosis of knee osteoarthritis
2. Taking anti-inflammatory and analgesic drugs in the past month
3. Taking blood-lipid-lowering drugs or health foods in the past month
4. Received any acupuncture treatment in the past 3 weeks

+Locations (1)

Facility	City	Country	Status
China Medical University	Taichung	Taiwan	Recruiting

+Investigators

Wan-Zhen Yu, Principal investigator, China Medical University, China