Whey vs Casein to Combat Post-inflammatory Protein and Muscle Waste - Combining Endotoxemia, Immobilisation and Fasting in Healthy Young Males in a New Model of Acute Febrile Disease
| Registry | NCT03319550 |
|---|---|
| Status | Completed |
| Phase | Not applicable |
| Study type | Interventional; randomized, crossover, masking: double, basic science |
| Enrollment | 10 (Actual) |
| Sponsor | University of Aarhus (Other); collaborators: Arla Foods |
| Conditions | Muscle Protein Synthesis, Endotoxemia, Nutrition, Milk Protein, Metabolism, Whey, Casein |
| Interventions | Casein, Whey, Leucine-enriched whey (Dietary supplement) |
| Dates | start 2017-12-07, primary completion 2018-09-19, completion 2018-09-19, first posted 2017-10-24, last updated 2019-04-25 |
| Locations | 1 site in Denmark |
Related Papers Related Patents Related Grants Related Experts
All trials for Muscle Protein Synthesis All trials for Endotoxemia All trials of Casein All trials of Whey
This study compares three different protein supplements (casein, whey and leucine-enriched whey) and their effect on post-inflammatory muscle waste in a model of acute disease. Each test person will undergo all three interventions.
It is believed that leucine is the primary driver of muscle protein synthesis and therefore we hypothesize that leucine-enriched whey and whey are superior to casein in combating post-inflammatory muscle waste, because of its higher leucine content (16%, 11% and 9% leucine, respectively).
Background:
Acute illness is accompanied by infection/inflammation, anorexia and immobilization all contributing to muscle loss, making nutritional supplement optimization an obvious target for investigation and eventually clinical intervention. In the clinical setting large heterogenicity among patients complicates investigations of muscle metabolism during acute illness. Therefore we introduce a disease model by combining "Inflammation + 36 hour fast and bedrest". Inflammation/febrile illness will be initiated by using the well-established "human endotoxemia model" with a bolus injection of Escherichia coli lipopolysaccharide (LPS), known to cause inflammation comparable with the initial phase of sepsis. The amino acid leucine has shown to be particularly anabolic in performance sports, but little is known about its potential beneficial effects during acute illness. Leucine is a powerful activator of muscle protein synthesis and it seems that protein supplements with the highest leucine content elicit a greater increase in protein synthesis than those with a smaller fraction of leucine.
The protein supplements used most in hospitals contain casein derived protein, which has a much lower leucine content than the whey protein compounds typically used in performance sports.
This study compares three different protein supplements.The study is an open, randomized crossover trial. Laboratory technicians, test subjects and investigators will be blinded.
Interventions:
I. LPS (1 ng/kg as bolus) + 36 h fasting + 36 h bedrest + Casein (9% leucine) II. LPS (1 ng/kg as bolus) + 36 h fasting + 36 h bedrest + Whey (11% leucine) III. LPS (1 ng/kg as bolus) + 36 h fasting + 36 h bedrest + Leucine-enriched whey (16% leucine)
The test objects will be given 0,6 g protein/kg, 1/3 as a bolus and 2/3 as sipping over a period of 3,5 hour. Muscle metabolism will be investigated by phenylalanine tracer using the forearm model and total protein metabolism using a carbamide tracer. Through muscle biopsies intracellular signalling pathways will be investigated.
| Arm | Type | Interventions | Description |
|---|---|---|---|
| Casein | Experimental | Dietary Supplement: Casein | "LPS + 36 hour fast and bedrest" + Casein (9% leucine) - 0.6 g protein/kg bodyweight, 1/3 as bolus and 2/3 as sipping. |
| Whey | Experimental | Dietary Supplement: Whey | "LPS + 36 hour fast and bedrest" + Whey (11% leucine) - 0.6 g protein/kg bodyweight, 1/3 as bolus and 2/3 as sipping |
| Leucine-enriched whey | Experimental | Dietary Supplement: Leucine-enriched whey | "LPS + 36 hour fast and bedrest" + Leucine-enriched whey (16% leucine) - 0.6 g protein/kg bodyweight, 1/3 as bolus and 2/3 as sipping |
Sex: Male, ages: 20 Years to 40 Years, accepts healthy volunteers
Inclusion Criteria: * Healthy Male * Age between 20-40 * BMI between 20-30 * Normal health examination and blood samples * Written informed consent Exclusion Criteria: * Immobilisation of an extremity, unless a doctor has declared it fully rehabilitated. * Allergy against lidocain or latex. * The use of anabolic steroids * Disease like: Diabetes, epilepsia, infection, cardiovascular disease.
| Facility | City | Country | Status |
|---|---|---|---|
| Aarhus University Hospital | Aarhus | Denmark |