The Use of Quantitative Pregnancy Test in Amniotic Fluid as a Diagnostic Tool for Rupture of Fetal Membranes

Beta subunit of human chorionic gonadotropin assay will be measured in the vaginal washing in two groups. The first group are pregnant women in the second or third trimester. These women have definite rupture of membranes diagnosed by visual leaking of the amniotic fluid from the cervix during speculum examination. The second group are pregnant women with intact membranes. Assessment of both the qualitative and quantitative beta subunit of human chorionic gonadotropin will be done

• assessment of beta subunit of human chorionic gonadotropin (Diagnostic test): Vaginal washing fluid will be collected and sent for measurement of beta subunit of human chorionic gonadotropin

Primary outcomes

• Concentration of beta subunit of human chorionic gonadotropin [This outcome will be measured within 20 minutes of collection of vaginal washing fluid. The collection of vaginal washing fluid will be done by an investigator upon admission to the department of Obstetrics]: Concentration of beta subunit of human chorionic gonadotropin will be measured in vaginal washing fluid. The collection of vaginal washing fluid will be done by an investigator

Secondary outcomes

• Presence or absence of positive qualitative pregnancy test [Qualitative pregnancy test will be done in the vaginal washing fluid by an investigator within 20 minutes from admission to the Obstetrics department.]: Qualitative pregnancy test will be done by an investigator in the Obstetrics department

Sex: Female, ages: 17 Years to 45 Years

Inclusion Criteria:

* Pregnant women between 14 and 40 weeks gestation with history of gush of vaginal fluid
* Pregnant women between without history of gush of vaginal fluid

Exclusion Criteria:

* Presence of vaginal bleeding
* Presence of any obstetric emergency as cord prolapse

• Eman Omran, M.D., Principal investigator, Cairo University