EIMD and Microgreens

NCT07774221 — Not applicable, Active not recruiting, 16 participants, Çanakkale Onsekiz Mart University

ClinicalTrials Daily · Areas · Conditions · Drugs · Sponsors ·

Sporcularda Yüksek Şiddetli Egzersiz Sonrası Mikro Yeşillerin Kullanımının Toparlanma Üzerine Etkilerinin İncelenmesi

Registry	NCT07774221, acronym Muscreen
Status	Active not recruiting
Phase	Not applicable
Study type	Interventional; randomized, parallel, masking: none, treatment
Enrollment	16 (Actual)
Sponsor	Çanakkale Onsekiz Mart University (Other)
Conditions	Exercise-Induced Muscle Stress
Interventions	Mixed Microgreens and Wheatgrass Juice, Lettuce (Dietary supplement)
Dates	start 2025-12-05, primary completion 2026-01-06, completion 2026-12-14 (estimated), first posted 2026-08-19, last updated 2026-08-19
Locations	1 site in Turkey (Türkiye)

-Summary

This study investigated whether a mixed microgreens intervention could modulate biochemical responses to strenuous eccentric exercise. The findings suggest that microgreens consumption was associated with lower post-exercise levels of several muscle damage and inflammatory biomarkers, particularly CK, LDH, AST, TNF-α, and CRP, while effects on myoglobin and IL-6 were limited. Further controlled studies are needed to confirm these findings and clarify their biological significance.

Expand all sections · Collapse all

+Arms and interventions

Arm	Type	Interventions	Description
Control group	Experimental	Dietary Supplement: Lettuce	Participants assigned to the control group received lettuce as the control green food and underwent the same exercise protocol, blood sampling schedule, and study procedures as the intervention group. Blood samples were collected at baseline and 24, 48, and 72 h after exercise.
Microgreens Intervention Group	Experimental	Dietary Supplement: Mixed Microgreens and Wheatgrass Juice	Participants received a mixed microgreens intervention consisting of black radish, red radish, broccoli, and kale microgreens at a total daily dose of 60 g, divided into three 20-g administrations consumed in the morning, at noon, and in the evening. Participants also consumed 30 mL of wheatgrass juice each morning. The intervention was administered over four cycles, with each cycle consisting of 5 consecutive days followed by a 2-day interval. The final intervention cycle coincided with the exercise period. Participants underwent the same standardized eccentric plyometric exercise protocol and blood sampling schedule as the control group.

Mixed Microgreens and Wheatgrass Juice (Dietary supplement): Participants received a mixed preparation of black radish, red radish, broccoli, and kale microgreens at a total daily dose of 60 g, divided into three 20-g administrations consumed in the morning, at noon, and in the evening. Participants also consumed 30 mL of wheatgrass juice each morning during the intervention period. The intervention was administered over four cycles, with each cycle consisting of 5 consecutive days followed by a 2-day interval. The final intervention cycle coincided with the exercise period.
Lettuce (Dietary supplement): Participants assigned to the control group received lettuce as the control green food according to the study protocol and underwent the same exercise and assessment procedures as the intervention group.

+Outcome measures

Primary outcomes

Serum biochemical responses following eccentric plyometric exercise [Baseline, 24, 48, and 72 hours after exercise]: Between-group differences in serum biochemical responses following the standardized high-intensity eccentric plyometric exercise protocol. Muscle-related biochemical markers included creatine kinase, lactate dehydrogenase, aspartate aminotransferase, and myoglobin. Inflammatory markers included interleukin-6, tumor necrosis factor-alpha, and C-reactive protein. Measurements were obtained at baseline and 24, 48, and 72 hours after exercise, and the temporal responses were compared between the control and plant-based intervention groups.

+Eligibility

Sex: Male, ages: 20 Years to 25 Years, accepts healthy volunteers

Inclusion Criteria:

* Male football players aged 20-25 years. Active participation in an amateur football team in Çanakkale, Türkiye. Physically active according to the physical activity criteria referenced by the American College of Sports Medicine and the World Health Organization.

No health condition that would prevent participation in the study procedures. Willingness to comply with the study protocol and scheduled assessments. Provision of written informed consent.

Exclusion Criteria:

* Musculoskeletal or joint injury, particularly involving the lower extremities, within the previous 6 months.

Regular use of medication. History of chronic disease, including cardiovascular disease, diabetes, or other metabolic disorders.

No regular participation in a structured training program during the preceding year.

History of alcohol or tobacco use. Inability or unwillingness to comply with the study procedures.

+Locations (1)

Facility	City	Country	Status
Çanakkale Onsekiz Mart University	Çanakkale	Turkey (Türkiye)