Effects of Strength Training Combined With Sustainable Source of Omega-3 Fatty Acids on Muscle Strength and Cognitive Functions in Adults Controlled Trial

NCT07763574 — Not applicable, Enrolling by invitation, 120 participants, Gdansk University of Physical Education and Sport

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Registry	NCT07763574
Status	Enrolling by invitation
Phase	Not applicable
Study type	Interventional; randomized, parallel, masking: double, basic science
Enrollment	120 (Estimated)
Sponsor	Gdansk University of Physical Education and Sport (Other)
Conditions	Muscle Strength, Cognitive Function Assessment
Interventions	Effects of Strength Training Combined With Omega-3s derived from algae on Muscle Strength and Cognitive Functions in adults, Effects of Strength Training Combined With Omega-3s derived from fish on Muscle Strength and Cognitive Functions in adults, Effects of Strength Training Combined With Placebo on Muscle Strength and Cognitive Functions in adults, Effects of Omega-3s derived from algae on Muscle Strength and Cognitive Functions in adults, Effects of Omega-3s derived from fish on Muscle Strength and Cognitive Functions in adults, Effects of Placebo on Muscle Strength and Cognitive Functions in adults (Dietary supplement)
Dates	start 2026-08-23 (estimated), primary completion 2029-01-01 (estimated), completion 2030-01-01 (estimated), first posted 2026-08-13, last updated 2026-08-13
Locations	1 site in Poland

-Summary

The purpose of the study is to evaluate the effects of 12-week supplementation with algae-derived DHA combined with strength training on muscle strength, neuromuscular parameters, post-exercise inflammation and cognitive functions in healthy adults compared to fish-derived EPA and DHA combined with strength training, strength training alone and control group.

The research methods and measurement tools include:

* Body composition assessed prior to the training intervention and after 12 weeks of training: body mass index (BMI) and body composition - fat mass \[kg\], body fat percentage \[%\] and skeletal muscle mass \[kg\] - will be determined using bioimpedance methods (In Body 720, Biospace, Seoul, Korea) following an overnight fast.
* Assessment of physical activity levels using the long version of the International Physical Activity Questionnaire (IPAQ).
* Assessment of mood (UMACL Adjective Mood Scale) and depression (DASS-21, Beck Depression Scale).
* The SarQoL (Sarcopenia and Quality of Life) questionnaire, used to specifically assess quality of life in people with reduced muscle mass and function.
* Assessment of participants' diet: each participant will be required to record their diet in 3-day food diaries (to be checked on the first day of testing).
* Blood sampling: blood will be collected from each participant at two time points before and after the intervention (on an empty stomach, and 40 minutes after the training session). The following will be collected: 1 × 6 ml blood count tube and 3 tubes with a clotting activator, with a total volume of 20 ml. Blood will be collected before the start of the training intervention and after 12 weeks of training. The tubes will be centrifuged at 4 °C (4000 × g for 10 minutes). The serum, plasma and erythrocyte fractions will then be frozen at -80 °C until analysis.
* Peak force, relative peak force and force at 150, 200 and 250 milliseconds during isometric mid-thigh pull-ups using a Kistler platform (Kistler dynamometer platform, model 9286BA, with MARS software). The maximum load (1RM) will also be measured for each participant. Upper limb grip strength will be measured using a SAEHAN electronic dynamometer.
* In addition, muscle electrical activity using a Noraxon Telemyo surface electromyograph will be assessed.
* Cognitive function will be assessed using the following tests: the Stroop Test, the Corsi Block-Tapping Task and the Pattern Separation Task.

All analysis (beside IPAQ) will be performed before and after 12 weeks of intervention.

* During the study, all participants will take part in a strength training session (around 40 mins duration) led by an experienced trainer to stimulate the muscles to release myokines.
* Blood tests will include: BDNF, IGF-1, IL-6, hs-CRP, TNF-alpha, and IL-1B as well as concentrations of metabolites of the kynurenine pathway.

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+Arms and interventions

Arm	Type	Interventions	Description
algae-derived DHA + strength training	Experimental	Dietary Supplement: Effects of Strength Training Combined With Omega-3s derived from algae on Muscle Strength and Cognitive Functions in adults	3g of a algae-derived DHA + strength training 3 times a week through 12 weeks
fish-derived omega-3 + strength training	Experimental	Dietary Supplement: Effects of Strength Training Combined With Omega-3s derived from fish on Muscle Strength and Cognitive Functions in adults	3g of a fish-derived omega-3 (EPA+DHA) + strength training 3 times a week through 12 weeks
placebo + strength training	Placebo comparator	Dietary Supplement: Effects of Strength Training Combined With Placebo on Muscle Strength and Cognitive Functions in adults	3g of a placebo + strength training 3 times a week through 12 weeks
algae-derived DHA without strength training	Active comparator	Dietary Supplement: Effects of Omega-3s derived from algae on Muscle Strength and Cognitive Functions in adults	3g of a algae-derived DHA through 12 weeks
fish-derived omega-3 without strength training	Active comparator	Dietary Supplement: Effects of Omega-3s derived from fish on Muscle Strength and Cognitive Functions in adults	3g of a fish-derived omega-3 (EPA+DHA) through 12 weeks
placebo without strength training	Placebo comparator	Dietary Supplement: Effects of Placebo on Muscle Strength and Cognitive Functions in adults	3g of a placebo through 12 weeks

Effects of Strength Training Combined With Omega-3s derived from algae on Muscle Strength and Cognitive Functions in adults (Dietary supplement): Healthy men and women with few years of training experience will participate in a 12-week strength training program combined with 3g/per day DHA from algae supplementation. The training will be 3 times a week, with 1-hour strength-training exercises for the upper and lower body. At the beginning and end of the intervention, muscle strength will be assessed along with EMG, cognitive function will be evaluated, and blood samples will be collected to measure specific proteins.
Effects of Strength Training Combined With Omega-3s derived from fish on Muscle Strength and Cognitive Functions in adults (Dietary supplement): Healthy men and women with few years of training experience will participate in a 12-week strength training program combined with EPA and DHA from fish supplementation (3g per day). The training will be 3 times a week, with 1-hour strength-training exercises for the upper and lower body. At the beginning and end of the intervention, muscle strength will be assessed along with EMG, cognitive function will be evaluated, and blood samples will be collected to measure specific proteins.
Effects of Strength Training Combined With Placebo on Muscle Strength and Cognitive Functions in adults (Dietary supplement): Healthy men and women with few years of training experience will participate in a 12-week strength training program combined with placebo supplementation. The training will be 3 times a week, with 1-hour strength-training exercises for the upper and lower body. At the beginning and end of the intervention, muscle strength will be assessed along with EMG, cognitive function will be evaluated, and blood samples will be collected to measure specific proteins.
Effects of Omega-3s derived from algae on Muscle Strength and Cognitive Functions in adults (Dietary supplement): Healthy, untrained men and women will participate in a 12-week DHA from algae supplementation (3g per day). At the beginning and end of the intervention, muscle strength will be assessed along with EMG, cognitive function will be evaluated, and blood samples will be collected to measure specific proteins.
Effects of Omega-3s derived from fish on Muscle Strength and Cognitive Functions in adults (Dietary supplement): Healthy, untrained men and women will participate in a 12-week EPA and DHA from fish supplementation (3g per day). At the beginning and end of the intervention, muscle strength will be assessed along with EMG, cognitive function will be evaluated, and blood samples will be collected to measure specific proteins.
Effects of Placebo on Muscle Strength and Cognitive Functions in adults (Dietary supplement): Healthy, untrained men and women will participate in a 12-week placebo supplementation. At the beginning and end of the intervention, muscle strength will be assessed along with EMG, cognitive function will be evaluated, and blood samples will be collected to measure specific proteins.

+Outcome measures

Primary outcomes

Peak force (N), relative peak force (N/kg bw) and force at 150, 200, and 250 milliseconds (N) during Isometric Mid-Thigh Pull (IMTP) and Back Squat [12 weeks intervention]

+Eligibility

Sex: All, ages: 25 Years to 35 Years, accepts healthy volunteers

Inclusion Criteria for the training group:

* 1-2 years of strength training experience,
* without chronic or autoimmune diseases,
* between the ages of 25 and 35,
* not obese,
* not taking omega-3 fatty acids,
* not taking protein, BCAAs, creatine, anabolic steroids, or other supplements
* for women: not pregnant or breastfeeding, and at least 1 year after childbirth (to allow for hormonal balance to stabilize).

For the control group (non-exercisers):

\- Requirements as above, except for exercise: we recruit individuals who exercise less than 1 hour per week

+Locations (1)

Facility	City	Country	Status
University of Physical Education and Sport	Gdansk	Poland

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