Evaluation of Bile Acid Concentrations and Their Signaling as Markers of Testicular Tumorigenesis and Germ Cell Tumor Chemosensitivity

• blood sampling (Biological): Blood samples will allow the measurement of AFP, LDH and HCG as well as the measurement of testosterone, glucose, cholesterol, triglycerides and bile acids
• Tissue sampling (Procedure): On these tissues (tumoral and non-tumoral), the investigators will carry out analysis of the bile acid composition by mass spectrometry, molecular characterization by RNAseq, histological characterization and analysis in cell culture

Primary outcomes

• To compare bile acid levels in healthy testicular tissues and in tumorous testicular tissues in patients with testicular germ cell tumor [During orchidectomy]: The investigators hypothesis is that bile acid levels are higher in tumor tissues compared to those present in healthy tissues of patients with testicular germ cell tumors, making them a marker of these tumors. Furthermore, the investigators suggest that blood bile acid levels and their composition are a relevant prognostic marker of these tumors.

Secondary outcomes

• Composition of bile acids in healthy and tumor testicular tissues [Perioperative]
• Correlation between blood bile acid levels and survival [From date of randomization until the date of first documented progression or date of death from any cause, whichever came first, assessed up to 100 months]
• Correlation between blood bile acid levels and the prognostic stage [From enrollment to 24 months after the end of treatment]
• Correlation between bile acid levels and tumor markers [From enrollment to 24 months after the end of treatment]
• Correlation bile acid levels kinetics with the response to chemotherapy [From enrollment to 24 months after the end of treatment]
• Correlation between bile acid level kinetics and survival [From enrollment to 24 months after the end of treatment]
• Analysis of chemosensitivity of tumor cells in vitro [From the end of chemotherapy to 24 months after the end of treatment]

Sex: Male, ages: 18 Years to any

Inclusion Criteria:

* Adult patient, male, with a clinical and biological diagnosis of germ cell tumor of the testis in pre-orchidectomy, at the stage of the disease and any histological subtype of germ cell tumor
* Affiliation with a Social Security organization
* Able to give informed consent to participate in the research.

Exclusion Criteria:

* Patient with a hepatobiliary pathology
* Patient treated with Colestyramine
* Patient who has undergone cholecystectomy or extensive resection of the small intestine
* Patient deprived of liberty
* Patient under guardianship, curatorship, or legal protection
* Patient unable to understand the protocol (language barrier, cognitive difficulties)
* Patient with another active cancer
* Patient participating in a therapeutic clinical trial
* Refusal to participate