Postprandial Triglyceride Concentrations Across Menstrual Cycle Phases

• Early Follicular Phase (Other): In the early follicular phase of the menstrual cycle, participants will undergo 6 hours of seated rest after the consumption of a high-fat meal representing 33% of estimated daily energy expenditure and consisting of 59% of calories from fat.
• Mid-Luteal Phase (Other): In the mid-luteal phase of the menstrual cycle, participants will undergo 6 hours of seated rest after the consumption of a high-fat meal representing 33% of estimated daily energy expenditure and consisting of 59% of calories from fat.

Primary outcomes

• Change from baseline in plasma total triglyceride concentrations [6 hours]: Plasma total triglyceride concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 30, 60, 90,120, 180, 240, 300, and 360 minutes).
• Change from baseline in plasma buoyant triglyceride concentrations [6 hours]: Plasma buoyant triglyceride concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 30, 60, 90,120, 180, 240, 300, and 360 minutes).
• Change from baseline in plasma denser triglyceride concentrations [6 hours]: Plasma denser triglyceride concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 30, 60, 90,120, 180, 240, 300, and 360 minutes).
• Change from baseline in plasma non-esterified fatty acid concentrations [6 hours]: Plasma non-esterified fatty acid concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 30, 60, 90,120, 180, 240, 300, and 360 minutes).
• Change from baseline in plasma insulin concentrations [6 hours]: Plasma insulin concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 30, 60, 90,120, 180, 240, 300, and 360 minutes).
• Change from baseline in plasma glucose concentrations [6 hours]: Plasma glucose concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 30, 60, 90,120, 180, 240, 300, and 360 minutes).
• Change from baseline in plasma β-hydroxybutyrate concentrations [6 hours]: Plasma β-hydroxybutyrate concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 60, 120, 180, 240, 300, and 360 minutes).

Secondary outcomes

• Baseline serum β-estradiol concentrations [Baseline]: Serum β-estradiol concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline)
• Baseline serum progesterone concentrations [Baseline]: Serum progesterone concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline)
• Fasting resting energy expenditure [Baseline]: Fasting resting energy expenditure will be measured via indirect calorimetry upon arrival at the laboratory (baseline).
• Change from baseline in respiratory exchange ratio [6 hours]: Respiratory exchange ratio will be measured via indirect calorimetry upon arrival at the laboratory (baseline), and hourly for 6 hours following meal consumption.
• Change from baseline in desire to eat [6 hours]: Scores of subjective desire to eat, measured using visual analog scales. Measurements are performed upon arrival at the laboratory (baseline) and hourly during the 6-hour exposure. Scores will be measured using semantic differential scales in the form of 100-mm visual analog scales. For desire to eat, the question will be asked as follows: "How strong is your desire to eat?" (very weak (0 mm) - very strong (100 mm)).
• Change from baseline in hunger [6 hours]: Scores of subjective hunger, measured using visual analog scales. Measurements are performed upon arrival at the laboratory (baseline) and hourly during the 6-hour exposure. Scores will be measured using semantic differential scales in the form of 100-mm visual analog scales. For hunger, the question will be asked as follows: "How hungry do you feel?" (not hungry at all (0 mm) - as hungry as I have ever felt (100 mm))
• Change from baseline in fullness [6 hours]: Scores of subjective fullness, measured using visual analog scales. Measurements are performed upon arrival at the laboratory (baseline) and hourly during the 6-hour exposure. Scores will be measured using semantic differential scales in the form of 100-mm visual analog scales. For fullness, the question will be asked as follows: How full do you feel?" (not full at all (0 mm) - very full (100 mm)).
• Change from baseline in prospective food consumption [6 hours]: Scores of subjective prospective food consumption, measured using visual analog scales. Measurements are performed upon arrival at the laboratory (baseline) and hourly during the 6-hour exposure. Scores will be measured using semantic differential scales in the form of 100-mm visual analog scales. For prospective food consumption, the question will be asked as follows: "How much food do you think you could eat?" (nothing at all (0 mm) - a large amount (100 mm)).
• Change from baseline in resting energy expenditure [6 hours]: Resting energy expenditure will be measured via indirect calorimetry upon arrival at the laboratory (baseline) and hourly for 6 hours following meal consumption. The thermic effect of food is defined as the increase in resting energy expenditure above the fasting baseline value.
• Change from baseline in carbohydrate oxidation rate [6 hours]: Carbohydrate oxidation rate will be measured via indirect calorimetry upon arrival at the laboratory (baseline) and hourly for 6 hours following meal consumption.
• Change from baseline in lipid oxidation rate [6 hours]: Carbohydrate oxidation rate will be measured via indirect calorimetry upon arrival at the laboratory (baseline) and hourly for 6 hours following meal consumption.

Sex: Female, ages: 18 Years to 30 Years, accepts healthy volunteers

Inclusion Criteria:

* English or French speaking
* Ability to provide informed consent

Exclusion Criteria:

* History or evidence of chronic disease
* Current use of hypolipemic medication
* Current use of hormonal contraceptives
* Current use of antidepressants
* Current use of anticoagulants
* Ongoing smoking status
* Experiencing pregnancy, puerperium, or irregular menstrual cycles