Postprandial Cardiometabolic Effects of Interrupting Sedentary Behavior With Exercise of Varying Intensity in Healthy Overweight Adults
| Registry | NCT07229963 |
|---|---|
| Status | Recruiting |
| Phase | Not applicable |
| Study type | Interventional; randomized, crossover, masking: none, prevention |
| Enrollment | 18 (Estimated) |
| Sponsor | Hacettepe University (Other) |
| Conditions | Cardiovascular Risk Markers, Postprandial Metabolism, Exercise Physiology, Substrate Oxidation |
| Interventions | Aerobic Exercise Intervention, Sprint Exercise Intervention, Sedentary Behaviour Intervention (Other) |
| Dates | start 2026-09-01, primary completion 2026-11-20 (estimated), completion 2026-12-15 (estimated), first posted 2025-11-17, last updated 2026-09-24 |
| Locations | 1 site in Turkey (Türkiye) |
Related Papers Related Patents Related Grants Related Experts
All trials for Cardiovascular Risk Markers All trials for Postprandial Metabolism All trials of Aerobic Exercise Intervention All trials of Sprint Exercise Intervention
The purpose of this study is twofold: (1) to determine whether breaking up prolonged sitting with aerobic or sprint cycling breaks reduces postprandial blood glucose, insulin, CRP, and blood pressure, which are established risk markers for cardiometabolic diseases; and (2) to assess substrate oxidation during this period in order to identify which exercise condition promotes the greatest increase in fat oxidation.
Sedentary behavior (SED) refers to activities such as sitting, lying down, or reclining that require energy expenditure of ≤1.5 MET during the time spent awake. Prolonged and continuous sedentary behavior leads to adverse effects such as abnormal postprandial metabolic responses, insulin resistance, vascular dysfunction, high blood pressure, increased carbohydrate oxidation, and inflammation. Epidemiological data also show that SED increases the risk of mortality, cardiovascular disease, type 2 diabetes, and certain types of cancer. Experimental studies have shown that 2-5 minutes of low- or moderate-intensity aerobic exercise performed every 20-30 minutes during the postprandial period improves cardiometabolic health by reducing glucose, insulin, and blood pressure during the postprandial period. On the other hand, it is known that short-term sprint interval exercise (SIE) increases insulin sensitivity by rapidly depleting muscle glycogen, improves glucose control, lowers blood pressure, and increases fat oxidation. With these effects, SIE may be a time-efficient method for interrupting SED. Although the cardiometabolic benefits of SIE and aerobic exercise interruptions (AEI) are known, there is a lack of studies comparing the effects of interrupting SED with these two exercise types of different intensities in preventing postprandial cardiometabolic dysfunction. In this context, the aim of the study is to compare the postprandial cardiometabolic effects of interrupting sedentary behavior with AIE and sprint exercise interruptions (SEI) in overweight but otherwise healthy women and men aged 30-45. In this randomized crossover trial, volunteers will participate in three trials: 1) AEI, 2) SEI, and 3) SED. There will be a minimum 4-day washout period between trials. In the SEI and AEI trials, exercise interruptions will be implemented every 30 minutes during the 7-hour sitting period, whereas in the SED trial, participants will remain seated continuously for the entire duration. In all conditions, two meals (breakfast and lunch) will be served 3 hours apart, and the trials will begin, after the breakfast meal is consumed. Venous blood samples for every 30 minutes of the first two hours following meals to measure glucose and to be taken hourly during the rest experiment will be used to analyze insulin, glucose, and C-reactive protein (CRP) levels. In addition, blood pressure will be measured hourly, and fat oxidation will be assessed at the baseline, 2nd, 5th, and 7th hours. The effects of the conditions on glucose and insulin will be evaluated using linear mixed models. A Two-Way (Trial x Number of Repeats) Analysis of Variance for Repeated Measures will be used to detect differences between conditions.
| Arm | Type | Interventions | Description |
|---|---|---|---|
| Sedentary: Participants will remain seated for 7 h. | Experimental | Other: Sedentary Behaviour Intervention | The sedentary condition will take place in a seated position. During the seated condition, volunteers will be seated at a typical desk chair at an appropriate height with hip and knee angles of \~90° and feet flat on the floor. The seating area will include a standard desk chair, a work desk, and the volunteers' personal laptops. Volunteers will be instructed prior to the visit to avoid behaviors such as fidgeting, excessive leg movements, and crossing their legs to minimize potential extraneous effects. |
| Breaking up sitting with aerobic exercise interruptions | Experimental | Other: Aerobic Exercise Intervention | The protocol that will interrupt sitting with AEI will involve cycle exercises performed at 50% of Wattmax for 2 minutes. The W and rpm levels corresponding to 50% of Wattmax will be determined using the values recorded during the determination of Wattmax. |
| Breaking up sitting with sprint exercise interruptions | Experimental | Other: Sprint Exercise Intervention | The protocol that will interrupt sitting with SEI will involve cycle exercises performed at the maximum revolutions per minute achievable within 10 seconds. Immediately (2-3 seconds) before each SEI, volunteers will increase their pedal cadence to the maximum speed they can achieve, after which a pedal load will be applied to the bicycle ergometer and a sprint will be performed against the applied load for 10 seconds. During each sprint, the pedal load will be applied at %200 Wattmax level. |
Sex: All, ages: 30 Years to 45 Years, accepts healthy volunteers
Inclusion Criteria * Being physically inactive (not doing at least 150 minutes/week of structured exercise). * Being sedentary (having a daily sedentary time/day for >7 hours). * Having a BMI between 25 and 29.9 kg/m2. * Having a history of regular menstrual cycles for female participants. * Having medical examination approval. Exclusion Criteria * Taking any acute or chronic medication or supplement that may affect metabolism. * Having an acute or chronic illness that limits exercise (musculoskeletal problems, cardiovascular disorders, respiratory problems, etc.). * Consuming tobacco products. * Meeting 3 or more of the metabolic syndrome criteria (waist circumference of 102 cm or more in men, 88 cm or more in women, triglyceride level of 150 mg/dL or higher, HDL cholesterol level below 40 mg/dL in men and 50 mg/dL in women, blood pressure of 130/85 mmHg or higher, fasting blood glucose level of 100 mg/dL (5.6 mg/L) or higher).
| Facility | City | Country | Status |
|---|---|---|---|
| Hacettepe Sports Science Faculty, Hacettepe Beytepe Clinic | Ankara, Beytepe | Turkey (Türkiye) | Recruiting |