Glycaemic Response and Insulinaemic Response for Nutralys S85 Plus and Whey Protein

A single-blind, randomised, repeat measure, crossover design trial will be used to study the glycaemic response and insulinaemic response following consumption of five products: one reference product (50 g available carbohydrate Glucose) and four test products (50 g available carbohydrate Glucose with 10 g NUTRALYS®S85 Plus pea protein, 50 g available carbohydrate Glucose with 20 g NUTRALYS®S85 Plus pea protein, 50 g available carbohydrate Glucose with 10 g Whey protein concentrate and 50 g available carbohydrate Glucose with 20 g Whey protein concentrate).

• 10 g NUTRALYS®S85 Plus pea protein (Dietary supplement): To determine the glycaemic response and insulinaemic response to 50g Glucose + 10 g NUTRALYS®S85 Plus pea protein.
• 20 g NUTRALYS®S85 Plus pea protein (Dietary supplement): To determine the glycaemic response and insulinaemic response to 50g Glucose + 20 g NUTRALYS®S85 Plus pea protein.
• 10 g Whey protein concentrate (Dietary supplement): To determine the glycaemic response and insulinaemic response to 50g Glucose + 10 g Whey protein concentrate.
• 20 g Whey protein concentrate (Dietary supplement): To determine the glycaemic response and insulinaemic response to 50g Glucose + 20 g Whey protein concentrate.
• 50 g Glucose (Dietary supplement): To determine the glycaemic response and insulinaemic response to 50g Glucose

Primary outcomes

• Change in blood glucose response from baseline for 3 hours [3 hours]: Change in postprandial blood glucose response from baseline was measured at 15 min, 30 min, 45 min, 1 hour, 1.5 hours, 2 hours, 2.5 hours and 3 hours
• Change in plasma insulin response from baseline for 3 hours [3 hours]: Change in postprandial plasma insulin response from baseline was measured at 15 min, 30 min, 45 min, 1 hour, 1.5 hours, 2 hours, 2.5 hours and 3 hours
• Area under the blood glucose concentration versus time curve [3 hours]: Area under the blood glucose concentration versus time curve was calculated by trapezoidal rule
• Area under the plasma insulin concentration versus time curve [3 hours]: Area under the plasma insulin concentration versus time curve was calculated by trapezoidal rule
• Peak blood glucose concentration (Cmax) [0-3 hours]: Peak blood glucose concentration (Cmax) after consumption of the test food
• Peak plasma insulin concentration (Cmax) [0-3 hours]: Peak plasma insulin concentration (Cmax) after consumption of the test food
• Time of blood glucose peak (Tmax) [0-3 hours]: Time of blood glucose peak after consumption of the test food
• Time of plasma insulin peak (Tmax) [0-3 hours]: Time of plasma insulin peak after consumption of the test food

Sex: All, ages: 18 Years to 60 Years, accepts healthy volunteers

Inclusion Criteria:

* Aged 18 to 60 years
* Body mass index (BMI) less than 30kg/m2

Exclusion Criteria:

* Pregnant or lactating
* Fasting blood glucose value > 6.1 mmol/l
* Any known food allergy or intolerance
* Medical condition(s) or medication(s) known to affect glucose regulation or appetite and/or which influence digestion and absorption of nutrients
* Known history of diabetes mellitus or the use of antihyperglycaemic drugs or insulin to treat diabetes and related conditions
* Major medical or surgical event requiring hospitalization within the preceding 3 months
* Use of steroids, protease inhibitors or antipsychotics (all of which have major effects on glucose metabolism and body fat distribution)
* Unable to comply with experimental procedures or did not follow GR testing safety guidelines.

• Siahanidou T, Margeli A, Kappis A, Papassotiriou I, Mandyla H. Circulating visfatin levels in healthy preterm infants are independently associated with high-density lipoprotein cholesterol levels and dietary long-chain polyunsaturated fatty acids. Metabolism. 2011 Mar;60(3):389-93. doi: 10.1016/j.metabol.2010.03.002. Epub 2010 Mar 31. PMID 20359723

• Informed Consent Form (PDF, 2020-08-25)

• Sangeetha Thondre, PhD, Principal investigator, Oxford Brookes University