House Dust Mite Induced Inflammasome Activation on Corticosteroid Resistance

NCT03307278 — Not applicable, Withdrawn, China Medical University Hospital

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Effects and Mechanism of House Dust Mite Induced Inflammasome Activation on Corticosteroid Resistance

Registry	NCT03307278
Status	Withdrawn - The research project cannot be implemented because the project has not received funding subsidies
Phase	Not applicable
Study type	Interventional; randomized, parallel, masking: none, basic science
Sponsor	China Medical University Hospital (Other)
Conditions	Allergic Asthma
Interventions	House dust mite (Biological)
Dates	start 2017-11-30 (estimated), primary completion 2018-09-06 (estimated), completion 2018-09-06 (estimated), first posted 2017-10-11, last updated 2021-08-02

-Summary

In this project, PBMCs will be isolated from HDM allergic and non allergic patient and then stimulated with HDM crude extract. The expression of inflammasome and the correlation of inflammasome activation and steroid resistance will be investigated.

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+Arms and interventions

Arm	Type	Interventions	Description
Steroid resistance in HDM allergic patients	Experimental	Biological: House dust mite
Steroid resistance in non HDM allergic subjects	Experimental	Biological: House dust mite

House dust mite (Biological): As a stimulant to induce inflammasome activation

+Outcome measures

Primary outcomes

inflammasome activation by HDM [6 hours]: The expression of NLRP3 in PBMCs will be measured by western blot after HDM stimulation.
Steroid receptor phosphorylation by HDM [6 hours]: The expression of steroid receptor will be detected by western blot and real-time PCR after HDM stimulation.

+Eligibility

Sex: All, ages: 20 Years to 70 Years, accepts healthy volunteers

Inclusion Criteria:

* HDM allergic and non allergic subjects

Exclusion Criteria:

* Pregnant subject, cancer