Non- Essential Amino Acid Requirements and Metabolism in Humans

NCT02009917 — Completed, 6 participants, University of British Columbia

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Application of Indicator Amino Acid Oxidation Technique to Determine Non-essential Amino Acids/Nitrogen Requirements in Adult Humans

Registry	NCT02009917
Status	Completed
Study type	Observational
Enrollment	6 (Actual)
Sponsor	University of British Columbia (Other); collaborators: Ajinomoto Co., Inc.
Conditions	Healthy Adult Males
Dates	start 2013-10-01, primary completion 2015-01-01, completion 2015-04-01, first posted 2013-12-12, last updated 2017-07-18
Locations	1 site in Canada

-Summary

Protein is a major structural component of all cells in the body. The nutritional importance of protein is because of their amino acids. 9 are called essential and 11 are called non-essential, based on whether we need to get them from diet. This classification is based on studies done using older techniques. Recent studies suggest that these so called non-essential amino acids play important roles in our body's health. For the first time, this proposal defines an experimental design to examine non- essential amino acid/nitrogen requirements and metabolism in humans using the Indicator Amino Acid Oxidation (IAAO) technique.

The objective of the current study is to examine the application of IAAO technique to determine the non-essential amino acid/nitrogen requirement in adult humans.

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+Outcome measures

Primary outcomes

13 Carbon dioxide production [8 hours (1study day)]: Urine and breath samples will be collected to measure the rate of oxidation of expired breath and flux by enrichment of urine.

+Eligibility

Sex: Male, ages: 20 Years to 40 Years, accepts healthy volunteers

Inclusion Criteria:

* 20-40 years of age
* Male
* Classified as normal body weight based on body mass index (BMI) 18.5-25kg/m2
* Free of any concurrent illness

Exclusion Criteria:

* Men not in good health or have a metabolic, neurological, genetic or immune disorder including diabetes and hypertension
* Men who are classified as underweight(<18.5kg/m2), overweight(25-30kg/m2) or obese (>30kg/m2) using the BMI classification)
* Men who are allergic to milk, eggs and egg protein

+Locations (1)

Facility	City	Country	Status
Child and Family Research Institute	Vancouver, British Columbia	Canada

+Publications

Results publication: Cooper L, Ball RO, Pencharz PB, Sakai R, Elango R. Dispensable Amino Acids, except Glutamine and Proline, Are Ideal Nitrogen Sources for Protein Synthesis in the Presence of Adequate Indispensable Amino Acids in Adult Men. J Nutr. 2020 Sep 1;150(9):2398-2404. doi: 10.1093/jn/nxaa180. PMID 32879983

+Investigators

Rajavel Elango, Ph.D, Principal investigator, University of British Columbia