Study to Determine the Immune Response From Broncho-alveolar Cells Against Pigeon Specific Antigens
| Registry | NCT01237145, acronym BIRD |
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| Status | Completed |
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| Study type | Observational |
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| Enrollment | 10 (Actual) |
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| Sponsor | Bossink, A.W.J. (Indiv) |
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| Conditions | Extrinsic Allergic Alveolitis |
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| Dates | start 2011-09-01, primary completion 2012-09-01, completion 2012-12-01, first posted 2010-11-09, last updated 2018-01-19 |
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| Locations | 1 site in Netherlands |
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Related Papers Related Patents Related Grants Related Experts
All trials for Extrinsic Allergic Alveolitis
-Summary
The purpose of this study is to establish if cells present in lung tissue during extrinsic allergic alveolitis (EAA) are able to elicit an immune response against specific antigens. The hypothesis is: cells in BAL lavage fluid from patients with EAA will be responsive to specific antigens and this response can be measured using ELISPOT.
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+Detailed description
Subjects suffering from extrinsic allergic alveolitis caused by pigeons will undergo a broncho alveolar lavage. Cells obtained from this BAL will be used for ELISPOT technique with pigeon specific antigens
+Arms and interventions
| Arm | Type | Interventions | Description |
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| non-EAA, pigeon | | | subject with BAL because of diseases not suspected to be EAA |
| EAA, pigeon | | | Bird fanciers with a typical clinical presentation suspected for pigeon induced EAA |
+Outcome measures
Primary outcomes
- ELISPOT response in cells obtained from broncho-alveolar lavage [2 days]: All elispots will be performed the same day, the end point will be available the next day
Secondary outcomes
- Elispot response in cells from blood [2 days]: ELispots will be performed the same day as blood collection, results will be available the next day
+Eligibility
Sex: All, ages: 18 Years to any
Inclusion Criteria:
* suspicion of pigeon induced extrinsic alveolitis
Exclusion Criteria:
* use of oral steroids
* unable to give informed consent
* age below 18
+Locations (1)
| Facility | City | Country | Status |
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| Diakonessenhuis Utrecht | Utrecht | Netherlands | |
+Publications
- Jafari C, Thijsen S, Sotgiu G, Goletti D, Dominguez Benitez JA, Losi M, Eberhardt R, Kirsten D, Kalsdorf B, Bossink A, Latorre I, Migliori GB, Strassburg A, Winteroll S, Greinert U, Richeldi L, Ernst M, Lange C; Tuberculosis Network European Trialsgroup. Bronchoalveolar lavage enzyme-linked immunospot for a rapid diagnosis of tuberculosis: a Tuberculosis Network European Trialsgroup study. Am J Respir Crit Care Med. 2009 Oct 1;180(7):666-73. doi: 10.1164/rccm.200904-0557OC. Epub 2009 Jul 9. PMID 19590020
- Losi M, Bossink A, Codecasa L, Jafari C, Ernst M, Thijsen S, Cirillo D, Ferrarese M, Greinert U, Fabbri LM, Richeldi L, Lange C; European Tuberculosis Network TBNET. Use of a T-cell interferon-gamma release assay for the diagnosis of tuberculous pleurisy. Eur Respir J. 2007 Dec;30(6):1173-9. doi: 10.1183/09031936.00067307. Epub 2007 Aug 22. PMID 17715165
- Kosters K, Nau R, Bossink A, Greiffendorf I, Jentsch M, Ernst M, Thijsen S, Hinks T, Lalvani A, Lange C. Rapid diagnosis of CNS tuberculosis by a T-cell interferon-gamma release assay on cerebrospinal fluid mononuclear cells. Infection. 2008 Dec;36(6):597-600. doi: 10.1007/s15010-007-7316-0. Epub 2008 Jan 12. PMID 18193383
+Investigators